diff --git a/Sources/CodeEditSourceEditor/Controller/TextViewController.swift b/Sources/CodeEditSourceEditor/Controller/TextViewController.swift
index fb95c81c1..96c6e2cd7 100644
--- a/Sources/CodeEditSourceEditor/Controller/TextViewController.swift
+++ b/Sources/CodeEditSourceEditor/Controller/TextViewController.swift
@@ -154,6 +154,9 @@ public class TextViewController: NSViewController {
     /// The type of highlight to use when highlighting bracket pairs. Leave as `nil` to disable highlighting.
     public var bracketPairEmphasis: BracketPairEmphasis? { configuration.appearance.bracketPairEmphasis }
 
+    /// The optional vertical separator between the gutter and editor content.
+    public var gutterSeparator: GutterSeparator? { configuration.appearance.gutterSeparator }
+
     /// The column at which to show the reformatting guide
     public var reformatAtColumn: Int { configuration.behavior.reformatAtColumn }
 
diff --git a/Sources/CodeEditSourceEditor/Gutter/GutterView.swift b/Sources/CodeEditSourceEditor/Gutter/GutterView.swift
index 8be57ec62..676501e5e 100644
--- a/Sources/CodeEditSourceEditor/Gutter/GutterView.swift
+++ b/Sources/CodeEditSourceEditor/Gutter/GutterView.swift
@@ -13,6 +13,27 @@ public protocol GutterViewDelegate: AnyObject {
     func gutterViewWidthDidUpdate()
 }
 
+/// Configures the vertical separator between the gutter and editor content.
+public struct GutterSeparator: Equatable {
+    /// The separator's stroke color.
+    public var color: NSColor
+    /// The separator's width in points.
+    public var lineWidth: CGFloat
+    /// Alternating painted and unpainted lengths. An empty array draws a solid line.
+    public var dashPattern: [CGFloat]
+
+    /// Creates a gutter separator.
+    /// - Parameters:
+    ///   - color: The separator's stroke color.
+    ///   - lineWidth: The separator's width in points.
+    ///   - dashPattern: Alternating painted and unpainted lengths. Leave empty for a solid line.
+    public init(color: NSColor = .separatorColor, lineWidth: CGFloat = 1, dashPattern: [CGFloat] = []) {
+        self.color = color
+        self.lineWidth = lineWidth
+        self.dashPattern = dashPattern
+    }
+}
+
 /// The gutter view displays line numbers that match the text view's line indexes.
 /// This view is used as a scroll view's ruler view. It sits on top of the text view so text scrolls underneath the
 /// gutter if line wrapping is disabled.
@@ -73,6 +94,14 @@ public class GutterView: NSView {
     @Invalidating(.display)
     var selectedLineColor: NSColor = NSColor.selectedTextBackgroundColor.withSystemEffect(.disabled)
 
+    var separator: GutterSeparator? {
+        didSet {
+            if oldValue != separator {
+                needsDisplay = true
+            }
+        }
+    }
+
     /// Toggle the visibility of the line fold decoration.
     @Invalidating(.display)
     public var showFoldingRibbon: Bool = true {
@@ -145,6 +174,7 @@ public class GutterView: NSView {
             controller: controller,
             delegate: delegate
         )
+        separator = configuration.appearance.gutterSeparator
     }
 
     public init(
@@ -327,6 +357,7 @@ public class GutterView: NSView {
         drawBackground(context, dirtyRect: dirtyRect)
         drawSelectedLines(context)
         drawLineNumbers(context, dirtyRect: dirtyRect)
+        drawSeparator(context)
         context.restoreGState()
     }
 
@@ -336,3 +367,18 @@ public class GutterView: NSView {
         textView = nil
     }
 }
+
+private extension GutterView {
+    func drawSeparator(_ context: CGContext) {
+        guard let separator, separator.lineWidth > 0 else { return }
+        context.saveGState()
+        context.setStrokeColor(separator.color.cgColor)
+        context.setLineWidth(separator.lineWidth)
+        context.setLineDash(phase: 0, lengths: separator.dashPattern)
+        let xPosition = bounds.maxX - separator.lineWidth / 2
+        context.move(to: CGPoint(x: xPosition, y: bounds.minY))
+        context.addLine(to: CGPoint(x: xPosition, y: bounds.maxY))
+        context.strokePath()
+        context.restoreGState()
+    }
+}
diff --git a/Sources/CodeEditSourceEditor/SourceEditorConfiguration/SourceEditorConfiguration+Appearance.swift b/Sources/CodeEditSourceEditor/SourceEditorConfiguration/SourceEditorConfiguration+Appearance.swift
index 6ca03f2a7..05f6c5b16 100644
--- a/Sources/CodeEditSourceEditor/SourceEditorConfiguration/SourceEditorConfiguration+Appearance.swift
+++ b/Sources/CodeEditSourceEditor/SourceEditorConfiguration/SourceEditorConfiguration+Appearance.swift
@@ -39,6 +39,9 @@ extension SourceEditorConfiguration {
         /// See ``BracketPairEmphasis`` for more information. Defaults to `.flash`.
         public var bracketPairEmphasis: BracketPairEmphasis? = .flash
 
+        /// The optional vertical separator between the gutter and editor content.
+        public var gutterSeparator: GutterSeparator?
+
         /// Create a new appearance configuration object.
         /// - Parameters:
         ///   - theme: The theme for syntax highlighting.
@@ -53,6 +56,7 @@ extension SourceEditorConfiguration {
         ///   - tabWidth: The visual tab width in number of spaces.
         ///   - bracketPairEmphasis: The type of highlight to use to highlight bracket pairs. See
         ///                          ``BracketPairEmphasis`` for more information. Defaults to `.flash`.
+        ///   - gutterSeparator: The optional vertical separator between the gutter and editor content.
         public init(
             theme: EditorTheme,
             useThemeBackground: Bool = true,
@@ -62,7 +66,8 @@ extension SourceEditorConfiguration {
             wrapLines: Bool,
             useSystemCursor: Bool = true,
             tabWidth: Int = 4,
-            bracketPairEmphasis: BracketPairEmphasis? = .flash
+            bracketPairEmphasis: BracketPairEmphasis? = .flash,
+            gutterSeparator: GutterSeparator? = nil
         ) {
             self.theme = theme
             self.useThemeBackground = useThemeBackground
@@ -77,6 +82,7 @@ extension SourceEditorConfiguration {
             }
             self.tabWidth = tabWidth
             self.bracketPairEmphasis = bracketPairEmphasis
+            self.gutterSeparator = gutterSeparator
         }
 
         @MainActor
@@ -123,6 +129,8 @@ extension SourceEditorConfiguration {
                 controller.emphasizeSelectionPairs()
             }
 
+            controller.gutterView.separator = gutterSeparator
+
             // Cant put these in one if sadly
             if #available(macOS 14, *) {
                 if oldConfig?.useSystemCursor != useSystemCursor {
diff --git a/Tests/CodeEditSourceEditorTests/Controller/TextViewControllerTests.swift b/Tests/CodeEditSourceEditorTests/Controller/TextViewControllerTests.swift
index bef6615bd..6808f2fea 100644
--- a/Tests/CodeEditSourceEditorTests/Controller/TextViewControllerTests.swift
+++ b/Tests/CodeEditSourceEditorTests/Controller/TextViewControllerTests.swift
@@ -458,6 +458,17 @@ final class TextViewControllerTests: XCTestCase {
         XCTAssertEqual(controller.textViewInsets.right, MinimapView.maxWidth)
     }
 
+    func test_gutterSeparator() {
+        XCTAssertNil(controller.gutterSeparator)
+        XCTAssertNil(controller.gutterView.separator)
+
+        let separator = GutterSeparator(color: .systemRed, lineWidth: 2, dashPattern: [1, 1])
+        controller.configuration.appearance.gutterSeparator = separator
+
+        XCTAssertEqual(controller.gutterSeparator, separator)
+        XCTAssertEqual(controller.gutterView.separator, separator)
+    }
+
     // MARK: Folding Ribbon
 
     func test_foldingRibbonToggle() {